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Sunday, February 27, 2022

Tomato Induces Cytotoxicity Against Cancer Cells, Scientists Suggest

Cytotoxicity is the process of the induction of cell death through toxins.

In other words, cell cytotoxicity is the process to use certain chemicals or mediator cells to destroy living cells.


In normal, cytotoxicity can cause the death of healthy cells


In cancers, cytotoxicity can be beneficial to humans by destroying the cancer cells and preventing them from proliferation.


For example, cytotoxicity induced by oxidative stress due to the imbalanced ratio of free radicals and antioxidant enzymes produced by the body can cause protein, lipid, and cellular damage that leads to chronic low-grade inflammatory diseases and cancer.

However, stimulation of ROS and antioxidants production to induce cytotoxicity to the tumors can also cause cancer cell death without damage to nearby healthy cells.

Dr. Shikha Srivastava, the lead scientist in the evaluation of the antioxidant and cytotoxic properties in Quercetin, wrote, "quercetin interacts with DNA directly, and could be one of the mechanisms for inducing apoptosis in both, cancer cell lines and tumor tissues by activating the intrinsic pathway".


And, " quercetin-induced cytotoxicity in leukemic cells in a dose-dependent manner, while ellagic acid showed only limited toxicity. Besides leukemic cells, quercetin also induced cytotoxicity in breast cancer cells, however, its effect on normal cells was limited or none".

Scientifically, most cases of cancer cells cytotoxicity are associated with the stimulation of protein and pathways involved in either gene suppression or cell death programming.


Tomato is red, edible fruit, genus Solanum, belonging to the family Solanaceae, native to South America. Because of its health benefits, the tomato is grown worldwide for the commercial purpose
and often in the greenhouse.

In the urgency to find a natural ingredient that kills cancer without inducing adverse effects, researchers examined the toxicity against human gastric adenocarcinoma cells (AGS) and the antioxidant activity of hydroethanolic (HME), acetone (AcE), and alkaloid (AE) extracts prepared from the leaves of the tomato plant.

Both HME and AcE extracts showed a significant effect in scavenging •NO (IC50 values ranged from 0.87 ± 0.12 to 1.54 ± 0.23 and from 0.90 ± 0.01 to 2.23 ± 0.14 mg mL-1, respectively), compared to HMEs that only scavenge O2•- showing IC50 values from 0.12 ± ≤0.01 to 0.43 ± 0.08 mg mL-1.


In other words, tomato extracts demonstrated a strong effect against human gastric adenocarcinoma cells (AGS) through their cytotoxicity without causing harm to normal cells through the antioxidant activity.

Furthermore, in the study to assess different varieties of tomato seed extracts potential antioxidant and/or antiproliferative activity, researchers found that San Marzano Rosso seed extract displays the highest free radical-scavenging activity with 68% of 2,2-Diphenyl-1-picrylhydrazyl radical inhibition.


Moreover, according to the brine shrimp test (LD50: 23,198 ppm), San Marzano Rosso seed extract also demonstrated the highest cytotoxicity against the A375 cell line, compared to other varieties.


Taken altogether, tomato and its leaves may be considered supplements for the prevention and treatment of cancer, pending the confirmation of the larger sample size and multicenter human study.


Intake of tomato in the form of supplement should be taken with extreme care to prevent overdose acute liver toxicity.

Natural Medicine for Fatty Liver And Obesity Reversal - The Revolutionary Findings To Achieve Optimal Health And Lose Weight

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Contrary To Professionals Prediction, Floaters Can Be Cured Naturally

Ovarian Cysts And PCOS Elimination
Holistic System In Existence That Will Show You How-To
Permanently Eliminate All Types of Ovarian Cysts Within 2 Months


Back to Kyle J. Norton Homepage http://kylejnorton.blogspot.ca


Author Biography
Kyle J. Norton (Scholar, Master of Nutrition, All right reserved)
Health article writer and researcher; Over 10.000 articles and research papers have been written and published online, including worldwide health, ezine articles, article base, health blogs, self-growth, best before it's news, the karate GB daily, etc.,.
Named TOP 50 MEDICAL ESSAYS FOR ARTISTS & AUTHORS TO READ by Disilgold.com Named 50 of the best health Tweeters Canada - Huffington Post
Nominated for shorty award over last 4 years
Some articles have been used as references in medical research, such as international journal Pharma and Bioscience, ISSN 0975-6299.

Sources
(1) Further insights on tomato plant: Cytotoxic and antioxidant activity of leaf extracts in human gastric cells by Figueiredo-González M1, Valentão P1, Pereira DM1, Andrade PB. (PubMed)
(2) Antioxidant and cytotoxic activities investigation of tomato seed extracts by Tommonaro G1, Caporale A, De Martino L, Popolo A, De Prisco R, Nicolaus B, Abbamondi GR, Saturnino C. (PubMed)
(3) Quercetin, a Natural Flavonoid Interacts with DNA, Arrests Cell. Cycle and Causes Tumor Regression by Activating Mitochondrial Pathway of Apoptosis by Shikha Srivastava, Ranganatha R. Somasagara, Mahesh Hegde, Mayilaadumveettil Nishana, Satish Kumar Tadi, Mrinal Srivastava, Bibha Choudhary & Sathees C. Raghavan. (Scientific Report)

Saturday, February 19, 2022

Cabbage May Prevent Damages Induced by Myocardial Infarction

Myocardial infarction is a severe medical emergency characterized by the obstruction of the blood supply to the brain, causing local brain neurons to die. Prompt treatment can minimize brain damage and potential post disease complications.


In other words, myocardial infarction (MI) occurs due to the imbalance in oxygen supply and demand caused by plaque rupture with thrombus formation in an artery, leading to reduced blood flow to the heart.

The occurrence and extent of brain infarction are associated with three basic factors, including the site of arterial occlusion, the rapidity of arterial occlusion, and the presence or absence of collateral circulation.

In other words, brain infarction can be classified into myocardial infarction due to plaque rupture and myocardial oxygen supply-demand imbalance secondary to other acute illnesses.

The exact causes of a blood clot to induce acute brain infarction are unknown. Plaques accumulation in the arteries associated with hardening and stiffing arterial walls is a leading cause of cardiovascular disease, including stroke.

According to the statistics provided by the CDC, every year, approximately, about 790,000 Americans have a heart attack, including 580,000 cases of a first heart attack and 210,000 to people who have already had a first heart attack.

The most common symptoms of MI are pressure, tightness, pain, or a squeezing or aching sensation in your chest, digestive discomforts such as nausea, indigestion, heartburn, abdominal pain, shortness of breath, and dizziness.

Conventionally, treatment of myocardial infarction is a focus to reduce the damage of the heart muscle accomplished through medical or mechanical means.

Cabbage is a species of Brassica oleracea belongings to the family Brassicaceae (or Cruciferae), native to the Mediterranean region along the seacoast. The veggie has a short stem with a crowded mass of leaves, usually green but in some varieties, it may be red or purplish.

On findings a natural whole food for the treatment of post-stroke, researchers examined the effects of anthocyanin-rich red cabbage extract (ARCE) on H2O2 treated rat neonatal cardiomyoblasts (H9c2 cells) and isoproterenol (ISO) induced rodent model of myocardial infarction.

According to the tested analysis,
* H2O2 treated H9c2 cells recorded cytotoxicity (48-50%) and apoptosis (57.3%), compared to the reduction in the presence of ARCE (7-10% & 12.3% respectively).


* Rats pretreated with ARCE for 30 days followed by ISO treatment recorded favorable heart: body weight ratio as compared to ISO treated group.

* Furthermore, the mRNA levels of enzymatic antioxidants (sod and catalase) and apoptotic genes (Bax and BCL-2) in the ARCE+ISO treated group was similar to the control group suggesting that ARCE pretreatment prevents ISO induced depletion of enzymatic antioxidants and apoptosis.

* Moreover, ARCE+ISO treated group (4.5%) recorded results comparable to control (0%) in infracted areas (10%) and derangement of the myocardium.


* ARCE+ISO treated group accounted promote the caveolin-3 associated with the heart muscle and SERCA2a expression involved in the reduced risk of heart failure, compared to the ISO treated group an indication of ARCE in the mediated reduction in membrane damage and calcium imbalance.

Based on the results, researchers wrote, "Overall this study elucidates the mechanism of ARCE mediated prevention of experimentally induced myocardial damage".

Taken altogether, cabbage may be used for the treatment of myocardial infarction, pending the confirmation of the larger sample size and multicenter human study.



Natural Medicine for Fatty Liver And Obesity Reversal - The Revolutionary Findings To Achieve Optimal Health And Lose Weight

How To Get Rid Of Eye Floaters
Contrary To Professionals Prediction, Floaters Can Be Cured Naturally

Ovarian Cysts And PCOS Elimination
Holistic System In Existence That Will Show You How-To
Permanently Eliminate All Types of Ovarian Cysts Within 2 Months

Back to Kyle J. Norton Homepage http://kylejnorton.blogspot.ca


Author Biography
Kyle J. Norton (Scholar, Master of Nutrition, All right reserved)
Health article writer and researcher; Over 10.000 articles and research papers have been written and published online, including worldwide health, ezine articles, article base, health blogs, self-growth, best before it's news, the karate GB daily, etc.,.
Named TOP 50 MEDICAL ESSAYS FOR ARTISTS & AUTHORS TO READ by Disilgold.com Named 50 of the best health Tweeters Canada - Huffington Post
Nominated for shorty award over last 4 years
Some articles have been used as references in medical research, such as international journal Pharma and Bioscience, ISSN 0975-6299.

Sources
(1) Anthocyanin rich extract of Brassica oleracea L. alleviates experimentally induced myocardial infarction by Jana S1, Patel D1,2, Patel S1, Upadhyay K1, Thadani J1, Mandal R3, Das S3, Devkar R. (PubMed)

Friday, February 4, 2022

Honey inhibits the Expression of Oxidative Stress with No Side Effects

Scientists may have found a natural alternative for the treatment of diseases associated with oxidative stress, according to studies.

Oxidative stress is a medical condition associated with the overexpression of free radicals compared to low levels of antioxidant enzymes produced by the host.

In fact, free radicals are constantly produced in animal and human cells. The air we breathe, the thing we eat, and the environment we contact are some factors that cause the production of reactive oxygen species (ROS).

Epidemiological studies suggested that oxidative stress can induce damage to the cell, lipid, proteins, and alternation of cell DNA, leading to chronic diseases, such as cardiovascular diseases, diabetes, and particularly, cancer.

How overexpression of free radicals causes damage to the cells. Researchers explained due to free radical unstable nature with unpair of electrons on the out ring of molecules, they constantly seek to donate or intercept the electron from other stable atoms, leading to a chain reaction that can not be stopped until all unpaired electrons are paired or inhibited by antioxidants.

These processes can lead to an imbalance of the ratio of free radicals and antioxidants produced by the host body resulting in oxidative stress, a major cause of cell, lipid, and protein damage.

In spite of free radical's harmful effects, one must understand that cellular oxygen species (ROS), are necessary with a moderate amount to protect our body in the mediation of antimicrobial infection, detoxification of potentially toxic compounds, and elimination of cancerous and other life-threatening cells through cytotoxicity.

On the other hand, antioxidants are stable molecules that inhibit oxidation. Antioxidants can reduce levels of free radicals and overexpression can limit the free radical function in carrying out their health benefits.

Therefore, in order to maintain good health, the levels of free radicals and antioxidants must be balanced.


Honey is a miraculous product made by bees using nectar from flowers.

The rich golden liquid is considered one of the healthy sweet food for replacing the use of white sugar and artificial sweetener by many people.

Researchers on finding a natural compound for the inhibition of oxidative stress examined the honey activity on oxidative stress induced by lead acetate exposure in male Wistar rats.

The study included 24 male Wistar rats randomly divided into four groups: Control group that received 1 ml/kg distilled orally for 28 days; while groups II-IV received 0.2% lead in drinking water and 1 ml/kg of distilled water, 1 ml/kg of honey, 1.5 ml/kg of honey respectively for 28 days.

Before pretreatment with honey, infected rats showed a decrease in locomotor and exploratory and decrease antioxidant activity increase in anxiety, memory impairment, lipid peroxidation.



Co-administration of honey with lead inhibited neurotoxicity observed by the improvement in memory function and decreased latency period and increased in time spent in target quadrant compared to the lead-exposed animals.


Injection of honey in the lead-fed group also increased locomotion, exploration, and decreased anxiety observed by the frequency of rearing, freezing duration, and the number of lines crossed by animals.

More importantly, the administration of honey improved the levels of antioxidant enzymes produced by the host observed by increased brain SOD, GST, and GSH activities compared to the lead-treated groups.


However, the honey pretreatment group showed no significant effect on MDA level, the oxidative stress marker.

These results suggested that honey exerted neuroprotective effects against lead-induced cognitive deficit probably by enhancing antioxidant activities without affecting the levels of MDA.



In order to reveal more information about honey antioxidative stress, researchers investigated the effects of Tualang honey (TH) protective activity against oxidative stress of exercise.

The study included 20 female athletes (aged 21.3 [2.1] years; body weight [BW] 54.1 [5.7] kg) were randomly assigned into two groups and consumed either 1.5 g/kg BW TH (high honey; HH; n = 10) or 0.75 g/kg BW TH (low honey; LH; n = 10).

Injection of Tualang honey showed a reduction of MDA in the LH group compared to the HH group. In other words, less oxidative stress was found in the LH group.


The concentrations of total phenolic content (TPC) and antioxidant activity (ferric reducing antioxidant power [FRAP]) increased from baseline to 2 and 1 h after TH consumption, respectively, then the concentrations returned toward baseline at 3 h in both LH and HH groups.


MDA concentration significantly decreased (p < 0.05) from baseline to 2 h and significantly increased from 2 to 3 h in the HH group. Meanwhile, ROS levels increased significantly from 0.5 to 3 h in the HH group.

Compared to the HH group, the LH group showed similar trends but insignificant increased levels of MDA and ROS.

Taken altogether, honey particularly Tualang honey may be considered functional remedies for the prevention and treatment of diseases related to the expression of oxidative stress with no side effects, pending the confirmation of a large sample size and multicenter human study



Natural Medicine for Fatty Liver And Obesity Reversal - The Revolutionary Findings To Achieve Optimal Health And Lose Weight

How To Get Rid Of Eye Floaters
Contrary To Professionals Prediction, Floaters Can Be Cured Naturally

Ovarian Cysts And PCOS Elimination
Holistic System In Existence That Will Show You How-To
Permanently Eliminate All Types of Ovarian Cysts Within 2 Months

Back to Kyle J. Norton Homepage http://kylejnorton.blogspot.ca



Author Biography
Kyle J. Norton (Scholar, Master of Nutrition, All right reserved)
Health article writer and researcher; Over 10.000 articles and research papers have been written and published online, including worldwide health, ezine articles, article base, health blogs, self-growth, best before it's news, the karate GB daily, etc.,.
Named TOP 50 MEDICAL ESSAYS FOR ARTISTS & AUTHORS TO READ by Disilgold.com Named 50 of the best health Tweeters Canada - Huffington Post
Nominated for shorty award over last 4 years
Some articles have been used as references in medical research, such as international journal Pharma ISSN 0975-6299.

Sources:
(1) Honey prevents neurobehavioural deficit and oxidative stress induced by lead acetate exposure in male Wistar rats- a preliminary study by Abdulmajeed WI1, Sulieman HB2, Zubayr MO2, Imam A3, Amin A2, Biliaminu SA4, Oyewole LA2, Owoyele BV. (PubMed)
(2) Dose-Response Effect of Tualang Honey on Postprandial Antioxidant Activity and Oxidative Stress in Female Athletes: A Pilot Study by Ahmad NS1, Abdul Aziz A2, Kong KW2, Hamid MSA3, Cheong JPG1, Hamzah SH1. (PubMed)
(3) The benefits and hazards of antioxidants: controlling apoptosis and other protective mechanisms in cancer patients and the human population by Salganik RI. (PubMed)