Posted by Chantel Martiromo. research contributed by PubMed
In the study to to screen various solvent extracts of whole plant of Torilis leptophylla
to display potent antioxidant activity in vitro and in vivo, total
phenolic and flavonoid contents in order to find possible sources for
future novel antioxidants in food and pharmaceutical formulations, posted in PubMed, showed that the total phenolic contents (TPC) (121.9±3.1 mg GAE/g extract) of TLM
while total flavonoid contents (TFC) of TLE (60.9 ±2.2 mg RTE/g extract)
were found significantly higher as compared to other solvent fractions.
Phytochemical screening of TLM revealed the presence of alkaloids,
anthraquinones, cardiac glycosides, coumarins, flavonoids, saponins,
phlobatannins, tannins and terpenoids. The EC50 values based on the DPPH
(41.0±1 μg/ml), ABTS (10.0±0.9 μg/ml) and phosphomolybdate (10.7±2
μg/ml) for TLB, hydroxyl radicals (8.0±1 μg/ml) for TLC, superoxide
radicals (57.0±0.3 μg/ml) for TLM and hydrogen peroxide radicals (68.0±2
μg/ml) for TLE were generally lower showing potential antioxidant
properties. A significant but marginal positive correlation was found
between TPC and EC50 values for DPPH, hydroxyl, phosphomolybdate and
ABTS, whereas another weak and positive correlation was determined
between TFC and EC50 values for superoxide anion and hydroxyl radicals.
Results of in vivo experiment revealed that administration of CCl4
caused a significant increase in lipid peroxidation (TBARS) while
decrease in GSH contents of liver. In contrast, TLM (200 mg/kg bw) and
silymarin (50 mg/kg bw) co-treatment effectively prevented these
alterations and maintained the antioxidant status.
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