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Thursday, December 18, 2014

Overcome Male Infertility - The perm-Ubiquitin tag immunoassay (SUTI)

Posted by Chantel Martiromo

Fertility is a natural process to insure the survival of human species. Through natural selection, we produce many offspring when the reproductive system works at it's peak in the suitable environment with plenty of food around. On the other hand, the reproductive system may completely shut down or work at it's minimum state and we produce less offspring, when the environment is hostile including less foods around, war, epidemic, but regardless of all these factors, most women are capable of conceiving during their menstrual cycle before menopause.

The test is to detect the inherited fertility disorder of the infertile male to avoid any unnecessary
fertility treatments for women.

I. Procedure
The semen sperm is taken from the infertile male in the clinic laboratory and the surface of sperm is screened for ubiquintin which is a small, highly-conserved regulatory protein that is ubiquitously expressed in complex structures enclosed within membranes.

II. Diagnosis
Since ubiquitnin is considered a universal marker of semen abnormalities, including sperm head and tail defects and semen contaminants such as spermatids, leukocytes and cellular debris.
The semen example is screened through immunofluorescence and flow cytometry, if there is an displays of increasing binding of anti-ubiquitin antibodies to sperm surface, the sample sperm is considered abnormality which increases the risk of infertility becasues elevated sperm ubiquitin levels have been associated with reduced fertility or infertility.

This test is still in developed state, if proven success, it not only helps to avoid many unnecessary treatments for unfertile couple but also helps to remove damaged sperm from semen sample before IVF and ICSC.
 
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